What is the purpose
of the feed analysis?

The feed analyses of the Tentamus labo­ra­to­ries can be used by large-scale producers, agri­cul­ture and smaller producers. The types of animal feed analysed include indi­vidual and compound feeds, basic feeds (maize silage, hay, etc.) and supple­men­tary feeds for live­stock, aqua­cul­ture, poultry, horses and pets. To ensure the safety and quality of these feed prod­ucts for animals and humans, chem­ical-phys­ical, micro­bi­o­log­ical, mole­c­ular biolog­ical, residue analyt­ical and sensory tests are used.

The range of feed analysis extends from simple guar­an­teed analysis assays to complete pack­ages with all para­me­ters used to prove nutri­tional adequacy according to regional and inter­na­tional stan­dards (e.g. AAFCO package (cat and dog food), antiox­i­dant package or GMO testing).

Regu­la­tion 767/2009 regu­lates the marketing and the use or feeding of feed.

How long does a feed analysis take?

The dura­tion of a feed analysis depends on the para­me­ters to be exam­ined and the product. We are happy to draw up indi­vidual analysis plans to meet your wishes and needs.

On average it takes 14 working days, but there are also analyses that are completed within 3 – 7 working days and for certain para­me­ters we also offer express analyses.

Crude protein, crude fat, crude fibre, crude ash – what is this?

Crude protein, crude fat, crude fibre and crude ash are typical compo­nents of feed­stuffs whose share of the total mass must be declared.

  • Crude protein: The protein contained in the feed is called crude protein. The supply of suffi­cient high-quality crude protein and its building blocks, the amino acids, is of vital impor­tance for animals. Since the body cannot produce all amino acids itself, the remaining essen­tial amino acids must be supplied through the diet. Accord­ingly, it is essen­tial that the animal is supplied with suffi­cient amino acids, i.e. crude protein. Crude protein is deter­mined and calcu­lated in the labo­ra­tory by means of the nitrogen it contains.
  • Crude fat: Crude fat is the fat contained in animal feed. Next to carbo­hy­drates, it is the most impor­tant energy supplier for the animals. There are essen­tial fatty acids that can only be supplied through the diet and are vital for the animals. The content of crude fat is deter­mined gravimetrically.
  • Crude fibre: Crude fibre includes all the dietary fibre contained in a feed product. Some animal species (e.g. horses, rabbits) depend on a minimum amount of crude fibre in their daily feed to ensure healthy diges­tion. Some feeds, such as wheat or maize silage, are well digestible in pigs and poultry in terms of crude fibre. Oats, on the other hand, have low digestibility in these species. The crude fibre content in feed is also deter­mined gravimetrically.
  • Crude ash: All inor­ganic compo­nents, such as minerals and trace elements in a feed, are referred to as crude ash. Crude ash can be deter­mined by burning a sample of feed at 550°C. The inor­ganic compo­nents remain. Only the inor­ganic compo­nents remain as ash. Their propor­tion is deter­mined by weighing.
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What is the
“Weender Analysis”?

Weender analysis is used in feed analysis to deter­mine the raw nutri­ents in a product and dates back to 1865.

Weender analysis is a quan­ti­ta­tive method that sepa­rates and exam­ines the feed into its indi­vidual compounds and raw nutrients:

  • Dry matter
  • Crude ash (potas­sium, sodium, iron, calcium etc.) [XA].
  • Crude protein [XP]
  • Crude fibre [XF]
  • Crude fat [XL]
  • Nitrogen-free extrac­tives (digestible carbo­hy­drates) [NfE]

These compo­nents must in turn be labeled on the feed pack­aging. The infor­ma­tion usually refers to the dry matter.

Proce­dure of the Weender feed analysis

  1. Deter­mi­na­tion of the raw water in the total feed (fresh matter) by drying at 105 °C for about two hours. Only the dry matter remains.
  2. Sepa­ra­tion of the dry matter into its indi­vidual compo­nents at 500°C. Inor­ganic compo­nents (raw ash) remain. Further processes can be used to sepa­rate this more closely into salt-soluble pure ash (bulk and trace elements) and insol­uble sili­cates (clay, sand).
  3. The organic compo­nents are deter­mined analyt­i­cally in each case (except NfE). 
    • Crude protein content: deter­mi­na­tion by their nitrogen content
    • Crude fat content: disso­lu­tion by organic solvents (e.g. hexane)
    • Crude fibre content: treat­ment of the sample with acid and alka­line solutions
    • Content of nitrogen-free extracts: Rest

With the Extended Weender Analysis“, which is also used in the labo­ra­to­ries of the Tentamus Group, raw fibres and NfE (nitrogen-free extrac­tives) can be deter­mined in more detail. This means that also fibres of the scaf­folding substances that are

  • not in neutral solu­tion (NDF)
  • not in acid solu­tion (ADF)
  • cannot be dissolved after increased acid treat­ment (ADL = lignin)

can be dissolved.

What other tests are used for feed analysis?

  • Microbiological analysis

    Feed can also contain pathogenic germs such as E.Coli or Listeria. These can be detected with the help of microbiological tests. If corresponding growth of these germs is observed, the products and production processes must be improved. The laboratories of the Tentamus Group examine the following germs:

    • Clostridia
    • Coli
    • Enterobacteriaceae
    • Listeria
    • Salmonella
    • Total plate count
    More information on
    Microbiological Analysis
    Kosmetik Analytik Mikrobio Preview DSC 5166
  • Molecular biological analysis

    In order to protect animals, certain animal components (whole carcasses, parts of carcasses of dead animals or products of animal origin including ova, embryos and semen not intended for breeding purposes) may not be fed in the EU. With the help of molecular biological analyses, feed can be tested for this (e.g. by means of PCR tests). In addition, the laboratories of the Tentamus Group also test whether feedstuffs declared as GMO-free are actually free of genetic engineering or whether they contain allergens.

    More information on
    Molecular Biological Analysis
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  • Chemical analysis

    Chemical analyses are carried out to examine the properties and ingredients of feed. The Weender analysis is used to examine the macronutrients in a feed product. In addition to the classic Weender analysis, we can also test feedstuffs for sugar, starch, sulphur, sweeteners, fluoride, chloride, fat ratios and much more.

    More information on
    Chemical Analysis
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  • Residue analysis

    Impurities such as pesticide residues, heavy metals or other contaminants can have a significant influence on the product and reduce its quality. Residue analyses can be used to determine the exact contents of undesirable substances, but also vitamin contents, in order to finally compare these with the legal maximum quantities. Our laboratories offer the following analyses for this purpose:

    • Aflatoxin B1
    • Amino acid profile
    • Antibiotic residues
    • Antioxidants
    • Calcium and phosphorus screening
    • Full GC/MS/MS pesticide screen
    • Full mineral profile
    • Dioxins, furans and dioxin-like PCBs
    • PAHs
    • PCBs
    • Fatty acid profile
    • FEDIAF alignment
    • Heavy metals
    • Melamine & related compounds
    • Cyanuric acid
    • Mycotoxins
    • Taurine
    • Ergot alkaloids
    • Solvent residues/ organic residues
    • Impurities Class I/ II/ III
    • Vitamin A, B, C, D, E, K
    • Biotin, folic acid
    • Trimethylamine
    • Betaine
    • Chlormequat and mepiquat chloride
    • Sulfonamide
    More information on
    Residue Analysis
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Legal basis
for feed analysis

Placing feed prod­ucts on the market is subject to a number of strict regu­la­tions that apply both nation­ally and at Euro­pean level. The following list provides an overview of the most impor­tant regu­la­tions and their contents in the EU:

Feed producers and oper­a­tors must observe these regu­la­tions as part of their due dili­gence and have them regu­larly checked by appro­priate experts.

Feed testing
in the laboratories of Tentamus

Tentamus Group has been providing quality assur­ance and R&D support services to the highly special­ized pet food industry for many years. With our global network of labo­ra­to­ries, we offer a range of rele­vant services such as:

  • Testing for impurities
  • Marketability assess­ment
  • Export compli­ance (certi­fi­ca­tion)
  • Labelling & regu­la­tory services
  • Micro­bi­ology
  • Quality manage­ment & auditing
  • Residue & pesti­cide analysis
  • Vita­mins

Our analyt­ical labo­ra­to­ries for feed analysis are ISO/IEC 17025 accred­ited and test according to the regu­la­tions in force in the respec­tive country.

Further­more, the Tentamus Group also includes labo­ra­to­ries that are GMP+ accred­ited. This enables and allows us to analyse certain crit­ical cont­a­m­i­nants in feed, feed addi­tives, premixes, compound feed and feed supple­ments for our customers.

In addi­tion, the Tentamus labo­ra­to­ries offer various pack­ages that are specif­i­cally requested in the feed sector:

  • AAFCO package (cat and dog food)
  • Aller­gens
  • Antiox­i­dants package
  • Biogenic Amines Package
  • Advanced treats/​pet food package
  • Glycol package
  • GMOs
  • Pet Food Screening
  • Probi­otic – Lactic Acid Bacteria Package
  • Stability studies
  • Vita­mins
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Analytics of animal food containing hemp

The use of hemp from commer­cial hemp (low in cannabi­noids) is becoming increas­ingly popular. Hemp seeds, hemp protein powder or other processed prod­ucts can be found on the market.

Since the different hemp vari­eties some­times contain consid­er­able amounts of narcotics, it is impor­tant to know the exact content of these substances.

As a compe­tent partner for hemp-containing prod­ucts, we ensure the safety of your prod­ucts and compli­ance with legal require­ments with our range of analyses. Our labo­ra­to­ries examine the following product groups:

  • Hemp treats (calming, mobility, health & well­ness boosters
  • Hemp oils, shampoo & pet cleaners
  • Dog chews & capsules for dogs
  • Catnip Spray
  • Hemp CBD Peanut Butter
  • Salmon Oil with Hemp
  • Balm
  • Hemp prod­ucts for horses

How can the
Tentamus service be integrated into your production process?

From the barn to the plate (or in the feed bowl) – the Tentamus Group offers you the complete range of prod­ucts from the analysis of feed and food prod­ucts in the labo­ra­tory, to advice on labeling, to auditing and hygiene advice for your produc­tion facility.

In doing so, we can offer you solu­tions indi­vid­u­ally adapted to your needs and support you with all your ques­tions on the subject of feed.

HACCP, audits, training

Feed producers are also subject to due dili­gence. They must be able to guar­antee and docu­ment the confor­mity of their prod­ucts and produc­tion facil­i­ties. In order to be able to safely face offi­cial hygiene audits, the expe­ri­enced and compe­tent audi­tors of the Tentamus Group offer you a service and consulting package for the devel­op­ment of HACCP concepts and quality manage­ment systems, imple­men­ta­tion of hygiene audits, as well as customised training for your company and your employees.

Laboratories for the Analysis Feed
of the Tentamus Group

The following labo­ra­to­ries of the Tentamus Group offer feed testing:

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